Tags

• stability 3
• neutrality 2
• lattice model 2
• protein 6
• mutation 1
• recombination 1
• mutagenesis 2
• library 2
• directed evolution 3
• error-prone PCR 1
• translation 16
• yeast 24
• translational robustness 3
• gene expression 8
• evolutionary rate 6
• codon usage 8
• E. coli 1
• regression 1
• noise 1
• mistranslation 6
• designability 1
• structure 1
• selection 4
• translational fidelity 4
• chaperone 1
• misfolding 4
• review 5
• evolution 7
• aggregation 11
• toxicity 1
• mass spectrometry 2
• fitness 1
• publication 23
• mRNA 2
• expression 1
• correlation 1
• Spearman 1
• attenuation 1
• RMA 1
• drosophila 1
• quinary 1
• engineering 1
• self-assembly 1
• fenex 1
• natural selection 2
• chemistry 2
• elongation 1
• high-throughput sequencing 1
• 5PSeq 1
• perspective 2
• data 2
• heat shock 14
• biochemistry 2
• disaggregation 1
• award 7
• RNA 7
• northern 1
• blot 1
• ribosome 1
• polysome 1
• gradient 1
• sequencing 1
• team 23
• 3D printing 1
• CBC 1
• Lackner 1
• collaboration 4
• HSF1 1
• prelim 1
• phase separation 3
• Pab1 3
• poly(A)-binding protein 1
• stress 8
• starvation 2
• proteomics 1
• student 9
• prize 2
• arnold 1
• sensing 1
• Sup35 1
• cGAS 1
• fellowship 7
• radioactivity 1
• liquid scintillation counting 1
• translation rate 1
• pH 3
• GFP 1
• flow cytometry 1
• intracellular 1
• transcription 1
• heat shock response 2
• Hsf1 3
• acidification 1
• birds 1
• cyanobacteria 1
• sedimentation 1
• condensation 4
• metabolism 1
• circadian 1
• doctorate 6
• PhD 6
• reporter 1
• luciferase 1
• amyloids 1
• stress granules 2
• pyruvate kinase 1
• funding 1
• NIH 1
• paper 14
• research 14
• chaperones 5
• condensates 2
• fluorescence anisotropy 1
• biomolecular condensation 6
• prizes 1
• LCR 1
• disorder 1
• Uversky 1
• charged region 1
• FCR 1
• ribosomal proteins 1
• nucleolus 1
• Sis1 1
• postdoc 1
• single molecule 1
• method 1
• quantification 1
• protein folding 1
• stress response 1
• HSR 1
• azide 2
• ethanol 2
• regulation 2
• article 1
• popular 1

stability

• Contact density affects protein evolutionary rate from bacteria to animals
• Predicting the tolerance of proteins to random amino acid substitution
• Thermodynamic prediction of protein neutrality

neutrality

• Predicting the tolerance of proteins to random amino acid substitution
• Thermodynamic prediction of protein neutrality

lattice model

• Predicting the tolerance of proteins to random amino acid substitution
• Thermodynamic prediction of protein neutrality

protein

• Single-molecule fluorescence multiplexing by multi-parameter spectroscopic detection of nanostructured FRET labels
• Stress-triggered phase separation is an adaptive, evolutionarily tuned response
• Reversible, specific, active aggregates of endogenous proteins assemble upon heat stress
• Estimating a structured covariance matrix from multi-lab measurements in high-throughput biology
• Predicting the tolerance of proteins to random amino acid substitution
• Thermodynamic prediction of protein neutrality

mutation

• On the conservative nature of intragenic recombination

recombination

• On the conservative nature of intragenic recombination

mutagenesis

• Why high-error-rate random mutagenesis libraries are enriched in functional and improved proteins
• On the conservative nature of intragenic recombination

library

• Why high-error-rate random mutagenesis libraries are enriched in functional and improved proteins
• On the conservative nature of intragenic recombination

directed evolution

• A Nobel Prize for evolution
• Why high-error-rate random mutagenesis libraries are enriched in functional and improved proteins
• On the conservative nature of intragenic recombination

error-prone PCR

• Why high-error-rate random mutagenesis libraries are enriched in functional and improved proteins

translation

• Chaperone regulation of biomolecular condensates
• Transcriptome-wide mRNA condensation precedes stress granule formation and excludes stress-induced transcripts
• Measuring translation in yeast using a PEST-nanoluciferase reporter
• Measuring translation in yeast by liquid scintillation counting
• Reversible, specific, active aggregates of endogenous proteins assemble upon heat stress
• Dying mRNA tells a story of its life
• Accounting for experimental noise reveals that mRNA levels, amplified by post-transcriptional processes, largely determine steady-state protein levels in yeast
• Stress, signaling, and biomolecular condensation
• A nutrient-driven tRNA modification alters translational fidelity and genome-wide protein coding across an animal genus
• Estimating selection on synonymous codon usage from noisy experimental data
• Good codons, bad transcript: large reductions in gene expression and fitness arising from synonymous mutations in a key enzyme
• Mistranslation-induced protein misfolding as a dominant constraint on coding-sequence evolution
• A diverse family of thermostable cytochrome P450s created by recombination of stabilizing fragments
• Population genetics of translational robustness
• A single determinant dominates the rate of yeast protein evolution
• Why highly expressed proteins evolve slowly

yeast

• An adaptive biomolecular condensation response is conserved across environmentally divergent species
• Measuring translation in yeast using a PEST-nanoluciferase reporter
• Transient intracellular acidification regulates the core transcriptional heat shock response
• Measuring translation in yeast by liquid scintillation counting
• Stress-triggered phase separation is an adaptive, evolutionarily tuned response
• Heat Shock Factor 1: from fire chief to crowd-control specialist
• A CBC Catalyst award to study asymmetric inheritance
• 3D printing yeast and stress granules
• RNATagSeq: multiplexed library preparation for Illumina RNA sequencing
• Yeast total RNA isolation protocol
• Reversible, specific, active aggregates of endogenous proteins assemble upon heat stress
• Supernatant/pellet fractionation of budding yeast cells by cryogenic lysis
• Disaggregation assay data visualization
• Aggregation assay data visualization
• Accounting for experimental noise reveals that mRNA levels, amplified by post-transcriptional processes, largely determine steady-state protein levels in yeast
• Stress, signaling, and biomolecular condensation
• Estimating a structured covariance matrix from multi-lab measurements in high-throughput biology
• Quantifying condition-dependent intracellular protein levels enables high-precision fitness estimates
• Estimating selection on synonymous codon usage from noisy experimental data
• Good codons, bad transcript: large reductions in gene expression and fitness arising from synonymous mutations in a key enzyme
• A diverse family of thermostable cytochrome P450s created by recombination of stabilizing fragments
• Structural determinants of the rate of protein evolution in yeast
• A single determinant dominates the rate of yeast protein evolution
• Why highly expressed proteins evolve slowly

translational robustness

• A diverse family of thermostable cytochrome P450s created by recombination of stabilizing fragments
• Population genetics of translational robustness
• Why highly expressed proteins evolve slowly

gene expression

• Accounting for experimental noise reveals that mRNA levels, amplified by post-transcriptional processes, largely determine steady-state protein levels in yeast
• Good codons, bad transcript: large reductions in gene expression and fitness arising from synonymous mutations in a key enzyme
• Misfolded proteins impose a dosage-dependent fitness cost and trigger a cytosolic unfolded protein response in yeast
• Mistranslation-induced protein misfolding as a dominant constraint on coding-sequence evolution
• A diverse family of thermostable cytochrome P450s created by recombination of stabilizing fragments
• Population genetics of translational robustness
• A single determinant dominates the rate of yeast protein evolution
• Why highly expressed proteins evolve slowly

evolutionary rate

• Contact density affects protein evolutionary rate from bacteria to animals
• A diverse family of thermostable cytochrome P450s created by recombination of stabilizing fragments
• Structural determinants of the rate of protein evolution in yeast
• Population genetics of translational robustness
• A single determinant dominates the rate of yeast protein evolution
• Why highly expressed proteins evolve slowly

codon usage

• Evolution of protein synthesis
• Protein misfolding and gene expression
• A nutrient-driven tRNA modification alters translational fidelity and genome-wide protein coding across an animal genus
• Estimating selection on synonymous codon usage from noisy experimental data
• Mistranslation-induced protein misfolding as a dominant constraint on coding-sequence evolution
• A diverse family of thermostable cytochrome P450s created by recombination of stabilizing fragments
• A single determinant dominates the rate of yeast protein evolution
• Why highly expressed proteins evolve slowly

E. coli

• A single determinant dominates the rate of yeast protein evolution

regression

• A single determinant dominates the rate of yeast protein evolution

noise

• A single determinant dominates the rate of yeast protein evolution

mistranslation

• A nutrient-driven tRNA modification alters translational fidelity and genome-wide protein coding across an animal genus
• How infidelity creates a sticky situation
• Signatures of protein biophysics in coding sequence evolution
• The evolutionary consequences of erroneous protein synthesis
• Mistranslation-induced protein misfolding as a dominant constraint on coding-sequence evolution
• Population genetics of translational robustness

designability

• Structural determinants of the rate of protein evolution in yeast

structure

• Structural determinants of the rate of protein evolution in yeast

selection

• Stress, signaling, and biomolecular condensation
• Quantifying condition-dependent intracellular protein levels enables high-precision fitness estimates
• Estimating selection on synonymous codon usage from noisy experimental data
• Contact density affects protein evolutionary rate from bacteria to animals

translational fidelity

• How infidelity creates a sticky situation
• Signatures of protein biophysics in coding sequence evolution
• The evolutionary consequences of erroneous protein synthesis
• Mistranslation-induced protein misfolding as a dominant constraint on coding-sequence evolution

chaperone

• Protein evolution: innovative chaps

misfolding

• Quantifying condition-dependent intracellular protein levels enables high-precision fitness estimates
• Misfolded proteins impose a dosage-dependent fitness cost and trigger a cytosolic unfolded protein response in yeast
• The evolutionary consequences of erroneous protein synthesis
• Protein evolution: innovative chaps

review

• Chaperone regulation of biomolecular condensates: new review!
• How infidelity creates a sticky situation
• Signatures of protein biophysics in coding sequence evolution
• The evolutionary consequences of erroneous protein synthesis
• Protein evolution: innovative chaps

evolution

• An adaptive biomolecular condensation response is conserved across environmentally divergent species
• Pervasive, conserved secondary structure in highly charged protein regions
• A Nobel Prize for evolution
• Evolution of protein synthesis
• Protein misfolding and gene expression
• The evolutionary consequences of erroneous protein synthesis
• Protein evolution: innovative chaps

aggregation

• Stress-triggered phase separation is an adaptive, evolutionarily tuned response
• Heat Shock Factor 1: from fire chief to crowd-control specialist
• A CBC Catalyst award to study asymmetric inheritance
• 3D printing yeast and stress granules
• Reversible, specific, active aggregates of endogenous proteins assemble upon heat stress
• Supernatant/pellet fractionation of budding yeast cells by cryogenic lysis
• Disaggregation assay data visualization
• Aggregation assay data visualization
• How infidelity creates a sticky situation
• Misfolded proteins impose a dosage-dependent fitness cost and trigger a cytosolic unfolded protein response in yeast
• Signatures of protein biophysics in coding sequence evolution

toxicity

• Misfolded proteins impose a dosage-dependent fitness cost and trigger a cytosolic unfolded protein response in yeast

mass spectrometry

• Chloroform-methanol extraction of proteins
• Quantifying condition-dependent intracellular protein levels enables high-precision fitness estimates

fitness

• Quantifying condition-dependent intracellular protein levels enables high-precision fitness estimates

publication

• Hsf1 and pH paper accepted
• New review on phase separation
• Jared Bard joins the lab
• Sammy Keyport joins the lab
• 'How heat kills cells'
• New paper on stress-triggered phase separation
• Haneul's a Ph.D. candidate
• HSF1 perspective in Molecular Cell
• Haneul Yoo joins the lab
• More coverage of our aggregation paper
• Coverage of our aggregation paper
• New paper on stress-induced protein aggregation
• Perspective in Cell
• Alexandra wins the L.J. Henderson thesis prize
• EMBO poster prize
• Alexandra wins Hoopes thesis prize
• New PLOS Genetics paper
• Edward awarded CBC postdoc grant
• Edward wins ASBMB poster prize
• New PLOS Biology paper
• BIG Seed award
• Chris Katanski joins the lab
• Josh wins an NSF fellowship

mRNA

• Single-molecule fluorescence multiplexing by multi-parameter spectroscopic detection of nanostructured FRET labels
• Estimating a structured covariance matrix from multi-lab measurements in high-throughput biology

expression

• Estimating a structured covariance matrix from multi-lab measurements in high-throughput biology

correlation

• Estimating a structured covariance matrix from multi-lab measurements in high-throughput biology

Spearman

• Estimating a structured covariance matrix from multi-lab measurements in high-throughput biology

attenuation

• Estimating a structured covariance matrix from multi-lab measurements in high-throughput biology

RMA

• Estimating a structured covariance matrix from multi-lab measurements in high-throughput biology

drosophila

• A nutrient-driven tRNA modification alters translational fidelity and genome-wide protein coding across an animal genus

quinary

• Stress, signaling, and biomolecular condensation

engineering

• Engineering self-assembling proteins

self-assembly

• Engineering self-assembling proteins

fenex

• Engineering self-assembling proteins

natural selection

• Evolution of protein synthesis
• Protein misfolding and gene expression

chemistry

• RNATagSeq: multiplexed library preparation for Illumina RNA sequencing
• Chloroform-methanol extraction of proteins

elongation

• Dying mRNA tells a story of its life

high-throughput sequencing

• Dying mRNA tells a story of its life

5PSeq

• Dying mRNA tells a story of its life

perspective

• Heat Shock Factor 1: from fire chief to crowd-control specialist
• Dying mRNA tells a story of its life

data

• Disaggregation assay data visualization
• Aggregation assay data visualization

heat shock

• Chaperone regulation of biomolecular condensates
• Transcriptome-wide mRNA condensation precedes stress granule formation and excludes stress-induced transcripts
• An adaptive biomolecular condensation response is conserved across environmentally divergent species
• HDX-MS finds that partial unfolding with sequential domain activation controls condensation of a cellular stress marker
• Using fluorescence anisotropy to monitor chaperone dispersal of RNA-binding protein condensates
• Chaperones directly and efficiently disperse stress-triggered biomolecular condensates
• Transient intracellular acidification regulates the core transcriptional heat shock response
• Stress-triggered phase separation is an adaptive, evolutionarily tuned response
• Heat Shock Factor 1: from fire chief to crowd-control specialist
• A CBC Catalyst award to study asymmetric inheritance
• 3D printing yeast and stress granules
• Reversible, specific, active aggregates of endogenous proteins assemble upon heat stress
• Disaggregation assay data visualization
• Aggregation assay data visualization

biochemistry

• Measuring translation in yeast by liquid scintillation counting
• Supernatant/pellet fractionation of budding yeast cells by cryogenic lysis

disaggregation

• Reversible, specific, active aggregates of endogenous proteins assemble upon heat stress

award

• Allan is a 2018 Scialog Fellow
• Allan is promoted with tenure
• Allan receives BIV junior faculty award
• Chris wins poster prize
• Josh wins a Harper Fellowship
• Allan receives Distinguished Investigator award
• Allan wins W.J. Youden award

RNA

• Chaperone regulation of biomolecular condensates
• Transcriptome-wide mRNA condensation precedes stress granule formation and excludes stress-induced transcripts
• Stressful steps: Progress and challenges in understanding stress-induced mRNA condensation and accumulation in stress granules
• RNATagSeq: multiplexed library preparation for Illumina RNA sequencing
• Yeast total RNA isolation protocol
• Sucrose gradient protocol for polysome profiles
• Anti-RNA blotting with chemiluminescent detection

northern

• Anti-RNA blotting with chemiluminescent detection

blot

• Anti-RNA blotting with chemiluminescent detection

ribosome

• Sucrose gradient protocol for polysome profiles

polysome

• Sucrose gradient protocol for polysome profiles

gradient

• Sucrose gradient protocol for polysome profiles

sequencing

• RNATagSeq: multiplexed library preparation for Illumina RNA sequencing

team

• Caitlin defends her thesis
• Chaperone regulation of biomolecular condensates: new review!
• Conservation of condensation paper featured!
• RNA condensation preprint
• Conservation of condensation paper published!
• Pab1 HDX paper accepted!
• Jared starts his own lab!
• Conservation of condensation preprint
• Hendrik defends his thesis
• Sammy defends her thesis
• Dana named IBD Scholar
• Josh wins BSCD fellowship
• Rosalind graduates summa cum laude
• Stress granule review published
• Haneul wins overall best dissertation
• Condensate dispersal protocol published
• Haneul wins best thesis prize
• Chaperone paper published
• Chaperone paper accepted
• NIH MIRA funds us for next 5 years
• Haneul Yoo defends her thesis
• Haneul passes her prelim
• Cat passes her prelim

3D printing

• 3D printing yeast and stress granules

CBC

• A CBC Catalyst award to study asymmetric inheritance

Lackner

• A CBC Catalyst award to study asymmetric inheritance

collaboration

• Pab1 HDX paper accepted!
• Single-molecule fluorescence multiplexing by multi-parameter spectroscopic detection of nanostructured FRET labels
• Daily cycles of reversible protein condensation in cyanobacteria
• A CBC Catalyst award to study asymmetric inheritance

HSF1

• Heat Shock Factor 1: from fire chief to crowd-control specialist

prelim

• Haneul passes her prelim

phase separation

• An adaptive biomolecular condensation response is conserved across environmentally divergent species
• Cellular sensing by phase separation: Using the process, not just the products
• Stress-triggered phase separation is an adaptive, evolutionarily tuned response

Pab1

• Using fluorescence anisotropy to monitor chaperone dispersal of RNA-binding protein condensates
• Cellular sensing by phase separation: Using the process, not just the products
• Stress-triggered phase separation is an adaptive, evolutionarily tuned response

poly(A)-binding protein

• Stress-triggered phase separation is an adaptive, evolutionarily tuned response

stress

• Chaperone regulation of biomolecular condensates
• Transcriptome-wide mRNA condensation precedes stress granule formation and excludes stress-induced transcripts
• Using fluorescence anisotropy to monitor chaperone dispersal of RNA-binding protein condensates
• Using fluorescence anisotropy to monitor chaperone dispersal of RNA-binding protein condensates
• Chaperones directly and efficiently disperse stress-triggered biomolecular condensates
• Cellular sensing by phase separation: Using the process, not just the products
• An Escherichia coli nitrogen starvation response is important for mutualistic coexistence with Rhodopseudomonas palustris
• Stress-triggered phase separation is an adaptive, evolutionarily tuned response

starvation

• An Escherichia coli nitrogen starvation response is important for mutualistic coexistence with Rhodopseudomonas palustris
• Stress-triggered phase separation is an adaptive, evolutionarily tuned response

proteomics

• An Escherichia coli nitrogen starvation response is important for mutualistic coexistence with Rhodopseudomonas palustris

student

• Caitlin defends her thesis
• Hendrik defends his thesis
• Sammy defends her thesis
• Dana named IBD Scholar
• Josh wins BSCD fellowship
• Haneul Yoo defends her thesis
• Cat Triandafillou defends her thesis
• Chris Katanski defends his thesis
• Josh Riback defends his thesis

prize

• Kyle wins the Grier Prize
• A Nobel Prize for evolution

arnold

• A Nobel Prize for evolution

sensing

• Cellular sensing by phase separation: Using the process, not just the products

Sup35

• Cellular sensing by phase separation: Using the process, not just the products

cGAS

• Cellular sensing by phase separation: Using the process, not just the products

fellowship

• Kyle wins F30
• Caitlin wins F31
• Hendrik wins F30
• Rosalind wins Liew Fellowship
• Sammy wins F31
• Jared wins Helen Hay Whitney fellowship
• Haneul wins F31

radioactivity

• Measuring translation in yeast by liquid scintillation counting

liquid scintillation counting

• Measuring translation in yeast by liquid scintillation counting

translation rate

• Measuring translation in yeast by liquid scintillation counting

pH

• HDX-MS finds that partial unfolding with sequential domain activation controls condensation of a cellular stress marker
• Transient intracellular acidification regulates the core transcriptional heat shock response
• Live cell measurement of the intracellular pH of yeast by flow cytometry using a genetically-encoded fluorescent reporter

GFP

• Live cell measurement of the intracellular pH of yeast by flow cytometry using a genetically-encoded fluorescent reporter

flow cytometry

• Live cell measurement of the intracellular pH of yeast by flow cytometry using a genetically-encoded fluorescent reporter

intracellular

• Live cell measurement of the intracellular pH of yeast by flow cytometry using a genetically-encoded fluorescent reporter

transcription

• Transient intracellular acidification regulates the core transcriptional heat shock response

heat shock response

• An adaptive biomolecular condensation response is conserved across environmentally divergent species
• Transient intracellular acidification regulates the core transcriptional heat shock response

Hsf1

• Chaperone regulation of biomolecular condensates
• Transcriptome-wide mRNA condensation precedes stress granule formation and excludes stress-induced transcripts
• Transient intracellular acidification regulates the core transcriptional heat shock response

acidification

• Transient intracellular acidification regulates the core transcriptional heat shock response

birds

• Transient intracellular acidification regulates the core transcriptional heat shock response

cyanobacteria

• Daily cycles of reversible protein condensation in cyanobacteria

sedimentation

• Daily cycles of reversible protein condensation in cyanobacteria

condensation

• Using fluorescence anisotropy to monitor chaperone dispersal of RNA-binding protein condensates
• Chaperones directly and efficiently disperse stress-triggered biomolecular condensates
• Reversible amyloids of pyruvate kinase couple cell metabolism and stress granule disassembly
• Daily cycles of reversible protein condensation in cyanobacteria

metabolism

• Daily cycles of reversible protein condensation in cyanobacteria

circadian

• Daily cycles of reversible protein condensation in cyanobacteria

doctorate

• Caitlin defends her thesis
• Hendrik defends his thesis
• Sammy defends her thesis
• Dana named IBD Scholar
• Josh wins BSCD fellowship
• Haneul Yoo defends her thesis

PhD

• Caitlin defends her thesis
• Hendrik defends his thesis
• Sammy defends her thesis
• Dana named IBD Scholar
• Josh wins BSCD fellowship
• Haneul Yoo defends her thesis

reporter

• Measuring translation in yeast using a PEST-nanoluciferase reporter

luciferase

• Measuring translation in yeast using a PEST-nanoluciferase reporter

amyloids

• Reversible amyloids of pyruvate kinase couple cell metabolism and stress granule disassembly

stress granules

• Stressful steps: Progress and challenges in understanding stress-induced mRNA condensation and accumulation in stress granules
• Reversible amyloids of pyruvate kinase couple cell metabolism and stress granule disassembly

pyruvate kinase

• Reversible amyloids of pyruvate kinase couple cell metabolism and stress granule disassembly

funding

• NIH MIRA funds us for next 5 years

NIH

• NIH MIRA funds us for next 5 years

paper

• Flipping the script on heat shock
• Chaperone regulation of biomolecular condensates: new review!
• Conservation of condensation paper featured!
• RNA condensation preprint
• Conservation of condensation paper published!
• Pab1 HDX paper accepted!
• Conservation of condensation preprint
• Rosalind graduates summa cum laude
• Stress granule review published
• Haneul wins overall best dissertation
• Condensate dispersal protocol published
• Haneul wins best thesis prize
• Chaperone paper published
• Chaperone paper accepted

research

• Flipping the script on heat shock
• Chaperone regulation of biomolecular condensates: new review!
• Conservation of condensation paper featured!
• RNA condensation preprint
• Conservation of condensation paper published!
• Pab1 HDX paper accepted!
• Conservation of condensation preprint
• Rosalind graduates summa cum laude
• Stress granule review published
• Haneul wins overall best dissertation
• Condensate dispersal protocol published
• Haneul wins best thesis prize
• Chaperone paper published
• Chaperone paper accepted

chaperones

• Chaperone regulation of biomolecular condensates: new review!
• An adaptive biomolecular condensation response is conserved across environmentally divergent species
• Using fluorescence anisotropy to monitor chaperone dispersal of RNA-binding protein condensates
• Using fluorescence anisotropy to monitor chaperone dispersal of RNA-binding protein condensates
• Chaperones directly and efficiently disperse stress-triggered biomolecular condensates

condensates

• Stressful steps: Progress and challenges in understanding stress-induced mRNA condensation and accumulation in stress granules
• Using fluorescence anisotropy to monitor chaperone dispersal of RNA-binding protein condensates

fluorescence anisotropy

• Using fluorescence anisotropy to monitor chaperone dispersal of RNA-binding protein condensates

biomolecular condensation

• Chaperone regulation of biomolecular condensates
• Transcriptome-wide mRNA condensation precedes stress granule formation and excludes stress-induced transcripts
• An adaptive biomolecular condensation response is conserved across environmentally divergent species
• HDX-MS finds that partial unfolding with sequential domain activation controls condensation of a cellular stress marker
• Adaptive preservation of orphan ribosomal proteins in chaperone-stirred condensates
• Stressful steps: Progress and challenges in understanding stress-induced mRNA condensation and accumulation in stress granules

prizes

• Sammy and Caitlin win poster prizes

LCR

• Pervasive, conserved secondary structure in highly charged protein regions

disorder

• Pervasive, conserved secondary structure in highly charged protein regions

Uversky

• Pervasive, conserved secondary structure in highly charged protein regions

charged region

• Pervasive, conserved secondary structure in highly charged protein regions

FCR

• Pervasive, conserved secondary structure in highly charged protein regions

ribosomal proteins

• Adaptive preservation of orphan ribosomal proteins in chaperone-stirred condensates

nucleolus

• Adaptive preservation of orphan ribosomal proteins in chaperone-stirred condensates

Sis1

• Adaptive preservation of orphan ribosomal proteins in chaperone-stirred condensates

postdoc

• Jared starts his own lab!

single molecule

• Single-molecule fluorescence multiplexing by multi-parameter spectroscopic detection of nanostructured FRET labels

method

• Single-molecule fluorescence multiplexing by multi-parameter spectroscopic detection of nanostructured FRET labels

quantification

• Single-molecule fluorescence multiplexing by multi-parameter spectroscopic detection of nanostructured FRET labels

protein folding

• HDX-MS finds that partial unfolding with sequential domain activation controls condensation of a cellular stress marker

stress response

• HDX-MS finds that partial unfolding with sequential domain activation controls condensation of a cellular stress marker

HSR

• An adaptive biomolecular condensation response is conserved across environmentally divergent species

azide

• Chaperone regulation of biomolecular condensates
• Transcriptome-wide mRNA condensation precedes stress granule formation and excludes stress-induced transcripts

ethanol

• Chaperone regulation of biomolecular condensates
• Transcriptome-wide mRNA condensation precedes stress granule formation and excludes stress-induced transcripts

regulation

• Chaperone regulation of biomolecular condensates
• Transcriptome-wide mRNA condensation precedes stress granule formation and excludes stress-induced transcripts

article

• Flipping the script on heat shock

popular

• Flipping the script on heat shock