Here you'll find protocols from others that we've found useful and tweaked to our liking, and our own protocols that we encourage others to use. Almost always a bit of both.
Using fluorescence anisotropy to monitor chaperone dispersal of RNA-binding protein condensates
tags: chaperones, condensates, Pab1, fluorescence anisotropy, stress
Measuring translation in yeast using a PEST-nanoluciferase reporter
by jared
tags: yeast, translation, reporter, luciferase
Measuring translation in yeast by liquid scintillation counting
tags: yeast, translation, biochemistry, radioactivity, liquid scintillation counting, translation rate
RNATagSeq: multiplexed library preparation for Illumina RNA sequencing
tags: yeast, RNA, sequencing, chemistry
Yeast total RNA isolation protocol
tags: yeast, RNA
Sucrose gradient protocol for polysome profiles
tags: RNA, ribosome, polysome, gradient
Anti-RNA blotting with chemiluminescent detection
tags: RNA, northern, blot
Supernatant/pellet fractionation of budding yeast cells by cryogenic lysis
tags: yeast, aggregation, biochemistry
Chloroform-methanol extraction of proteins
tags: chemistry, mass spectrometry